류현모 Hyun-Mo Ryoo   교수 / D.D.S., Ph.D.
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· Major : 분자유전학, 전사조절학, 후성유전학
· E-mail : hmryoo@snu.ac.kr
· Tel : 02-880-2320
· Laboratory : 골형성 전사조절 연구실 홈페이지
Current Research Interests

뼈를 형성을 조절하는 조골세포, 상아질의 형성을 조절하는 상아모세포에서 광화 (mineralization)와 연관된 유전자의 발현을 조절하는 전사인자와 그들의 조절 기전을 연구하여, 뼈형성이 필요한 골다공증, 치주질환 등의 새로운 치료방법을 개발하고자 함


 

Research Methodology

1. 유전자적중 마우스를 이용한 유전자 기능연구
2. Runx2, Dlx5, Osx, Msx2 등 골형성 관련 전사인자 활성연구
3. 골형성, 골광화 촉진물질 screening system
4. PIN1에 의한 RUNX2 단백질의 Posttranslational modification (PTM): 활성 강화, 안정성 증가

Publication / Patent
Publications
1: Kim WJ, Kim BS, Cho YD, Yoon WJ, Baek JH, Woo KM, Ryoo HM. Fibroin particle-supported cationic lipid layers for highly efficient intracellular protein delivery. Biomaterials. 2017 Apr; 122:154-162. 
2: Cho Y, Kim B, Bae H, Kim W, Baek J, Woo K, Lee G, Seol Y, Lee Y, Ku Y, Rhyu I, Ryoo H. Direct Gingival Fibroblast/Osteoblast Transdifferentiation via Epigenetics. J Dent Res. 2017 Jan 1 [Epub ahead of print]
3: Bae HS, Yoon WJ, Cho YD, Islam R, Shin HR, Kim BS, Lim JM, Seo MS, Cho SA, Choi KY, Baek SH, Kim HG, Woo KM, Baek JH, Lee YS, Ryoo HM. An HDAC Inhibitor, Entinostat/MS-275, Partially Prevents Delayed Cranial Suture Closure in Heterozygous Runx2 Null Mice. J Bone Miner Res. 2017 Jan 4. [Epub ahead of print] 
4: Kim WJ, Islam R, Kim BS, Cho YD, Yoon WJ, Baek JH, Woo KM, Ryoo HM. Direct Delivery of Recombinant Pin1 Protein Rescued Osteoblast Differentiation of Pin1 Deficient Cells. J Cell Physiol. 2016 Nov 1. [Epub ahead of print] 
5: Cho YD, Kim BS, Lee CS, Kim KH, Seol YJ, Lee YM, Rhyu IC, Ku Y, Ryoo HM. Fibronectin-Derived Oligopeptide Stimulates Osteoblast Differentiation Through a BMP2-Like Signaling Pathway. J Periodontol. 2016 Sep 13:1-13.
6: Cho YD, Bae HS, Lee DS, Yoon WJ, Woo KM, Baek JH, Lee G, Park JC, Ku Y, Ryoo HM. Epigenetic Priming Confers Direct Cell Trans-Differentiation From Adipocyte to Osteoblast in a Transgene-Free State. J Cell Physiol. 2016 Jul; 231(7):1484-94.
7: Islam R, Yoon WJ, Ryoo HM. Pin1, the Master Orchestrator of Bone Cell Differentiation. J Cell Physiol. 2016 May 25. [Epub ahead of print]
8: Shin HR, Islam R, Yoon WJ, Lee T, Cho YD, Bae HS, Kim BS, Woo KM, Baek JH, Ryoo HM. Pin1-mediated Modification Prolongs the Nuclear Retention of β-Catenin in Wnt3a-induced Osteoblast Differentiation. J Biol Chem. 2016 Mar 11; 291(11):5555-65.
9: Lim KE, Park NR, Che X, Han MS, Jeong JH, Kim SY, Park CY, Akiyama H, Kim JE, Ryoo HM, Stein JL, Lian JB, Stein GS, Choi JY. Core binding factor β of osteoblasts maintains cortical bone mass via stabilization of Runx2 in mice. J Bone Miner Res. 2015 Apr; 30(4):715-22.
10: Yoon WJ, Islam R, Cho YD, Ryu KM, Shin HR, Woo KM, Baek JH, Ryoo HM. Pin1 plays a critical role as a molecular switch in canonical BMP signaling. J Cell Physiol. 2015 Mar; 230(3):640-7.
11: Woo KM, Jung HM, Oh JH, Rahman SU, Kim SM, Baek JH, Ryoo HM. Synergistic effects of dimethyloxalylglycine and butyrate incorporated into α-calcium sulfate on bone regeneration. Biomaterials. 2015 Jan; 39:1-14.
12: Islam R, Bae HS, Yoon WJ, Woo KM, Baek JH, Kim HH, Uchida T, Ryoo HM. Pin1 regulates osteoclast fusion through suppression of the master regulator of cell fusion DC-STAMP. J Cell Physiol. 2014 Dec; 229(12):2166-74.
13: Yoon H, Kim HL, Chun YS, Shin DH, Lee KH, Shin CS, Lee DY, Kim HH, Lee ZH, Ryoo HM, Lee MN, Oh GT, Park JW. NAA10 controls osteoblast differentiation and bone formation as a feedback regulator of Runx2. Nat Commun. 2014 Nov 7;5:5176.
14: Lee DS, Choung HW, Kim HJ, Gronostajski RM, Yang YI, Ryoo HM, Lee ZH, Kim HH, Cho ES, Park JC. NFI-C regulates osteoblast differentiation via control of osterix expression. Stem Cells. 2014 Sep;32(9):2467-79.
15: Cho YD, Yoon WJ, Kim WJ, Woo KM, Baek JH, Lee G, Ku Y, van Wijnen AJ, Ryoo HM. Epigenetic modifications and canonical wingless/int-1 class (WNT) signaling enable trans-differentiation of nonosteogenic cells into osteoblasts. J Biol Chem. 2014 Jul 18;289(29):20120-8
16: Islam R, Yoon WJ, Woo KM, Baek JH, Ryoo HM. Pin1-mediated prolyl isomerization of Runx1 affects PU.1 expression in pre-monocytes. J Cell Physiol. 2014 Apr;229(4):443-52.
17: Yoon WJ, Cho YD, Kim WJ, Bae HS, Islam R, Woo KM, Baek JH, Bae SC, Ryoo HM. Prolyl isomerase Pin1-mediated conformational change and subnuclear focal accumulation of Runx2 are crucial for fibroblast growth factor 2 (FGF2)-induced osteoblast differentiation. J Biol Chem. 2014 Mar 28;289(13):8828-38.
18: Yoon WJ, Islam R, Cho YD, Woo KM, Baek JH, Uchida T, Komori T, van Wijnen A, Stein JL, Lian JB, Stein GS, Choi JY, Bae SC, Ryoo HM. Pin1-mediated Runx2 modification is critical for skeletal development. J Cell Physiol. 2013 Dec;228(12):2377-85.
19: Cho YD, Kim WJ, Yoon WJ, Woo KM, Baek JH, Lee G, Kim GS, Ryoo HM. Wnt3a stimulates Mepe, matrix extracellular phosphoglycoprotein, expression directly by the activation of the canonical Wnt signaling pathway and indirectly through the stimulation of autocrine Bmp-2 expression. J Cell Physiol. 2012 Jun;227(6):2287-96.
20: Kim HN, Lee JH, Bae SC, Ryoo HM, Kim HH, Ha H, Lee ZH. Histone deacetylase inhibitor MS-275 stimulates bone formation in part by enhancing Dhx36-mediated TNAP transcription. J Bone Miner Res. 2011 Sep;26(9):2161-73.
21: Kim BG, Lee JH, Yasuda J, Ryoo HM, Cho JY. Phospho-Smad1 modulation by nedd4 E3 ligase in BMP/TGF-β signaling. J Bone Miner Res. 2011 Jul;26(7):1411-24.
22: Seul KJ, Cho HS, Heo SH, Baek WY, Kim JE, Park EK, Choi JY, Ryoo HM, Cho JY. Osteoblast-specific expression of MEF induces osteopenia through downregulation of osteoblastogenesis and upregulation of osteoclastogenesis. J Bone Miner Res. 2011 Feb;26(2):341-50.
23: Jun JH, Yoon WJ, Seo SB, Woo KM, Kim GS, Ryoo HM, Baek JH. BMP2-activated Erk/MAP kinase stabilizes Runx2 by increasing p300 levels and histone acetyltransferase activity. J Biol Chem. 2010 Nov 19;285(47):36410-9
24: Cho YD, Yoon WJ, Woo KM, Baek JH, Park JC, Ryoo HM. The canonical BMP signaling pathway plays a crucial part in stimulation of dentin sialophosphoprotein expression by BMP-2. J Biol Chem. 2010 Nov 19;285(47):36369-76.
25: Jang JY, Park EK, Ryoo HM, Shin HI, Kim TH, Jang JS, Park HS, Choi JY, Kwon TG. Polymorphisms in the Matrilin-1 gene and risk of mandibular prognathism in Koreans. J Dent Res. 2010 Nov;89(11):1203-7.
26: Song GA, Kim HJ, Woo KM, Baek JH, Kim GS, Choi JY, Ryoo HM. Molecular consequences of the ACVR1(R206H) mutation of fibrodysplasia ossificans progressiva. J Biol Chem. 2010 Jul; 285(29):22542-53.
27: Park OJ, Kim HJ, Woo KM, Baek JH, Ryoo HM. FGF2-activated ERK mitogen-activated protein kinase enhances Runx2 acetylation and stabilization. J Biol Chem. 2010 Feb 5; 285(6):3568-74.
28: Cho YD, Yoon WJ, Woo KM, Baek JH, Lee G, Cho JY, Ryoo HM. Molecular regulation of matrix extracellular phosphoglycoprotein expression by bone morphogenetic protein-2. J Biol Chem. 2009 Sep 11;284(37):25230-40. 
29: Yoon WJ, Cho YD, Cho KH, Woo KM, Baek JH, Cho JY, Kim GS, Ryoo HM. The Boston-type craniosynostosis mutation MSX2 (P148H) results in enhanced susceptibility of MSX2 to ubiquitin-dependent degradation. J Biol Chem. 2008 Nov 21;283(47):32751-61. 
30: Kim BG, Kim HJ, Park HJ, Kim YJ, Yoon WJ, Lee SJ, Ryoo HM, Cho JY. Runx2 phosphorylation induced by fibroblast growth factor-2/protein kinase C pathways. Proteomics. 2006 Feb;6(4):1166-74. 
31: Ryoo HM, Lee MH, Kim YJ. Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cells. Gene. 2006 Jan 17;366(1):51-7. Review. 
32: Lee MH, Kim YJ, Yoon WJ, Kim JI, Kim BG, Hwang YS, Wozney JM, Chi XZ, Bae SC, Choi KY, Cho JY, Choi JY, Ryoo HM. Dlx5 specifically regulates Runx2 type II expression by binding to homeodomain-response elements in the Runx2 distal promoter. J Biol Chem. 2005 Oct 21;280(42):35579-87. 
33: Kim YJ, Lee MH, Wozney JM, Cho JY, Ryoo HM. Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2. J Biol Chem. 2004 Dec 3;279(49):50773-80. 
34: Lee MH, Kwon TG, Park HS, Wozney JM, Ryoo HM. BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2. Biochem Biophys Res Commun. 2003 Sep 26;309(3):689-94. 
42: Kim HJ, Kim JH, Bae SC, Choi JY, Kim HJ, Ryoo HM. The protein kinase C pathway plays a central role in the fibroblast growth factor-stimulated expression and transactivation activity of Runx2. J Biol Chem. 2003 Jan 3;278(1):319-26. 
35: Park MH, Shin HI, Choi JY, Nam SH, Kim YJ, Kim HJ, Ryoo HM. Differential expression patterns of Runx2 isoforms in cranial suture morphogenesis. J Bone Miner Res. 2001 May;16(5):885-92. 
36: Lee MH, Javed A, Kim HJ, Shin HI, Gutierrez S, Choi JY, Rosen V, Stein JL, van Wijnen AJ, Stein GS, Lian JB, Ryoo HM. Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor beta1 in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not sufficient for osteoblast differentiation. J Cell Biochem. 1999 Apr 1;73(1):114-25.