Rohidas B. Arote Rohidas B. Arote   Assistant Professor / PhD
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· Major : Gene Therapy
· E-mail : rohi06@snu.ac.kr
· Tel : 02-740-8770
· Laboratory : Biopolymer and Gene Therapy Laboratory
Current Research Interests

1. Gene therapy

  • Synthesis and characterization of biodegradable polymer-based gene delivery system
  • Chemical modifications of polymers for cell-specific targeting (e.g. liver, tumor, dental tissue), hydrophilicity and high transfection efficiency
  • Nanoparticle formulations for therapeutic delivery agents
  • Plasmid DNA, RNA isolation, purification, in vitro cell study and in vivo animal study      
  • Application of gene therapy in oral cancer and dentistry

 



2. Drug delivery system         

  • Preparation and characterizations of controlled release drug carrier.
  • Chemical modification for cell specificity, hydorphilicity, and mucoadhesive property
  • Research (Formulation Development) New Drug Delivery Systems.

 



3. Tissue Engineering

·         Synthesis and characterization of biomaterials and its in vitro evaluation

·         Conductive approach, tissue induction, cell transplantation

·         Organ transplant

·         Engineering salivary gland function

Research Methodology
Gene Therapy, Drug Delivery, Tissue Engineering
Publication / Patent
Jiang HL, Kim YK, Arote RB, Choi YJ, Park IK, Cho MH, Cho CS, Chitosan derivatives as DNA and siRNA carriers, Carbohyd polym (Submitted) Islam MA, Yun CH, Choi YJ, Shin JY, Arote R, Jiang HL, Kang SK, Nah JW, Park IK, Cho MH, Cho CS. Accelerated gene transfer through a polysorbitol-based transporter mechanism. Biomaterials. 2011 Dec;32(36):9908-24. Islam MA, Jiang HL, Quan JS, Arote RB, Kang ML, Yoo HS, Yun CH, Choi YJ, Cho CS. Mucoadhesive and pH-sensitive thiolated Eudragit microspheres for oral delivery of Pasteurella multocida antigens containing dermonecrotoxin. J Nanosci Nanotechnol. 2011 May;11(5):4174-81. Arote RB, Jiang HL, Kim YK, Cho MH, Choi YJ, Cho CS. Degradable poly(amido amine)s as gene delivery carriers. Expert Opin Drug Deliv. 2011 Sep;8(9):1237-46. Jiang HL, Lim HT, Kim YK, Arote R, Shin JY, Kwon JT, Kim JE, Kim JH, Kim D, Chae C, Nah JW, Choi YJ, Cho CS, Cho MH. Chitosan-graft-spermine as a gene carrier in vitro and in vivo, Eur J Pharm Biopharm. 2011 Jan;77(1):36-42. Kim YK, Kwon JT, Choi JY, Jiang HL, Arote R, Jere D, Je YH, Cho MH, Cho CS, Suppression of tumor growth in xenograft model mice by programmed cell death 4 gene delivery using folate-PEG-baculovirus. Cancer Gene Ther. 2010 Nov;17(11):751-60. Arote RB, Jere D, Cho CS, Structure activity relationship of poly(ester amine)s as gene carriers. Mater Technol (In Press) Jiang HL, Kim YK, Lee SM, Park MR, Kim EM, Jin YM, Arote R, Jeong HJ, Song SC, Cho MH, Cho CS, Galactosylated chitosan-g-PEI/DNA complexes-loaded poly(organophosphazene) hydrogel as a hepatocyte targeting gene delivery system. Arch Pharm Res. 2010; 33(4):551-6. Park IK, Singha K, Arote RB, Choi YJ, Kim WJ, Cho CS, pH sensitive polymers as gene carriers. Macromol Rapid Comm. 2010: 31 Arote RB, Hwang SK, Lim HT, Kim TH, Jere D, Jiang HL, Kim YK, Cho MH, Cho CS, Improved therapeutic response in a xenograft mice model for TAM67 gene via folate receptor mediated endocytosis, Biomaterials 2010; 31(8): 2435-2445. Arote RB, Lee ES, Hwang SK, Jere D, Jiang HL, Kim YK, Choi YJ, Cho MH, Cho CS, Efficient gene delivery with osmotically active, hyperbranched poly (ester amine)s, Bioconjug Chem. 2009; 20(12):2231-41. Jiang HL, Xu CX, Kim YK, Arote R, Jere D, Cho MH, Cho CS, The suppression of lung tumorigenesis by aerosol-delivered folate-chitosan-graftpolyethylenimine/Akt1 shRNA complexes through the Akt signaling pathway, Biomaterials 2009; 30(29):5844-52. Jere D, Jiang H, Arote R, Kim Y, Choi Y, Cho M, Akaike T, Cho C Degradable polyethylenimines as DNA and small interfering RNA carriers. Expert Opin Drug Deliv. 2009; 6(8):827-34. Jere D, Jiang HL, Kim YK, Arote R, Choi YJ, Yun CH, Cho MH, Cho CS. Chitosan-graft-polyethylenimine for Akt1 siRNA delivery to lung cancer cells. Int J Pharm. 2009; 13:378(1-2):194-200. Arote RB, Yoo MK, Kim TH, Jere D, Jiang HL, Kim YK, Cho MH, Cho CS, Folate Conjugated poly (ester amine) for lung cancer therapy, J Nanosci Nanotechnol. 2010; 10(5): 3294-8. Jere D, Arote RB, Jiang HL, Kim YK, Cho MH and Cho CS, Biodegradable Nano-Polymeric System for Efficient Akt1 siRNA, J Nanosci Nanotechnol. 2010; 10 (5): 3366-9. Jiang HL, Kim YK, Arote R, Jere D, Quan JS, Yu JH, Choi YJ, Nah JW, Cho MH, Cho CS. Mannosylated chitosan-graft-polyethylenimine as a gene carrier for Raw 264.7 cell targeting. Int J Pharm. 2009; 375(1-2):133-139. Arote RB, Jere D, Jiang HL, Kim YK, Cho MH, Cho CS, Biodegradable poly(ester amine)s for gene delivery applications. Biomed mater. 2009; 4(4): 44102. Jere D, Arote R, Jiang HL, Kim YK, Cho MH, Cho CS, Bioreducible polymers for efficient gene and siRNA delivery. Biomed mater. 2009; 4(2):25020. Kim YK, Choi JY, Jiang HL, Arote R, Jere D, Cho MH, Je YH, Cho CS. Hybrid of baculovirus and galactosylated PEI for efficient gene carrier. Virology. 2009 387(1):89-97. Arote RB, Hwang SK, Yoo MK, Jere D, Jiang HL, Kim YK, Choi YJ, Nah JW, Cho MH, Cho CS. Biodegradable poly(ester amine) based on glycerol dimethacrylate and polyethylenimine as a gene carrier. J Gene Med. 2008; 10(11):1223-35. Arote RB, Jere D, Cho CS, Biodegradable polyester derivatives as gene carrier. Current Trends in Polymer Science. 2008; 12: 1-17. Jiang HL, Arote RB, Jere D, Kim YK, Cho MH, Cho CS, Degradable polyethylenimines as gene carriers, Materials Science and Technology, 2008; 24(9):1118-1126. Jere D, Kim JE, Arote RB, Jiang HL, Kim YK, Choi YJ, Yun CH, Cho MH, Cho CS, Akt1 silencing efficiencies in lung cancer cells by sh/si/ssiRNA transfection using a reductable polyspermine carrier, Biomaterials. 2009; 30(8):1635-47. Jiang HL, Kwon JT, Kim EM, Kim YK, Arote R, Jere D, Jeong HJ, Jang MK, Nah JW, Xu CX, Park IK, Cho MH, Cho CS. Galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine as a gene carrier for hepatocyte-targeting. J Control Release. 2008; 131(2):150-7. Jere D, Xu CX, Arote R, Yun CH, Cho MH, Cho CS. Poly(beta-amino ester) as a carrier for si/shRNA delivery in lung cancer cells. Biomaterials. 2008; 29(16):2535-47. Choi MK, Arote R, Kim SY, Chung SJ, Shim CK, Cho CS, Kim DD. Transfection of primary human nasal epithelial cells using a biodegradable poly (ester amine) based on polycaprolactone and polyethylenimine as a gene carrier. J Drug Target. 2007; 15(10):684-90. Jere D, Yoo MK, Arote R, Kim TH, Cho MH, Nah JW, Choi YJ, Cho CS. Poly (amino ester) composed of poly (ethylene glycol) and aminosilane prepared by combinatorial chemistry as a gene carrier. Pharm Res. 2008; 25(4):875-85. Kim YK, Choi JY, Yoo MK, Jiang HL, Arote R, Je YH, Cho MH, Cho CS. Receptor-mediated gene delivery by folate-PEG-baculovirus in vitro. J Biotechnol. 2007; 131(3):353-61. Jiang HL, Kwon JT, Kim YK, Kim EM, Arote R, Jeong HJ, Nah JW, Choi YJ, Akaike T, Cho MH, Cho CS. Galactosylated chitosan-graft-polyethylenimine as a gene carrier for hepatocyte targeting. Gene Ther. 2007; 14(19):1389-98. Guo DD, Moon HS, Arote R, Seo JH, Quan JS, Choi YJ, Cho CS. Enhanced anticancer effect of conjugated linoleic acid by conjugation with Pluronic F127 on MCF-7 breast cancer cells. Cancer Lett. 2007; 254(2):244-54.